Figure 1.

Assembly of the filter trapping apparatus

Schematic of the assembly of a cellulose acetate membrane and supporting filter papers in a slot blot cassette. Pre-soak both of the membrane and filter papers with 1% SDS solution. Place the cellulose acetate membrane on top of the filter papers. (Left, side view) Arrange this assembly above the bottom part of the slot-blot cassette (Gasket support plate) of the filter trapping apparatus and clamp this setup to the top part of cassette (sample template) with screws and tighten them well under vacuum connected to the vacuum. (Right, top view) Schematic view of the tightened cassette ready for sample loading into its well for the filter trapping experiment.