| Reagent | Final concentration | Amount |
|---|---|---|
| Opti-MEM | n/a | 96-X μL |
| FuGENE HD | n/a | 4 μL |
| Plasmid: pCDNA5/FRT/TO, pCDNA5/FRT/TO-FTH1, pCDNA5/FRT/TO-FTH1∗, pCDNA5/FRT/TO-p27, pCDNA5/FRT/TO-p27∗, pCAGEN-FLAG-GFP, or pCAGEN-GFP–TDP-43ΔNLS | 0.01 μg/μL | X μL (1 μg) |
| Total | n/a | 100 μL |
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