| Reagent | Final concentration | Amount |
|---|---|---|
| 0.1× anti-mouse IgG antibody conjugated to IRDye 800CW or 0.1× anti-rabbit IgG antibody conjugated to IRDye 800CW | 1:10000 | 4 μL |
| Odyssey Blocking Buffer (TBS) | n/a | 4 mL |
| 20% Tween-20 | 0.2% | 40 μL |
| Total | n/a | 4044 μL |
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