15% Optiprep

Reagent	Final concentration	Amount
Molecular bio Grade H2O	–	7.5 mL
10× DPBS (no ca/mg)	1×	1 mL
Optiprep	15%	1.5 mL
Total	n/a	–

Avoid prolonged light exposure, store at 4°C for up to 2 wks.