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. 2022 Jul 22;13:4236. doi: 10.1038/s41467-022-31912-1

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The recovery of fluorescence intensity in cells expressing I₂-hDLG::GFP (A) and I₃-hDLG::GFP (D) treated with w7, Tg, w7 + Tg, and Tg + Bapta fit by a single-phase exponential growth curve. N = 8 cells; ROI = 13 (Tg), 10 cells; ROI = 22 (Tg + w7), 9 cells; ROI = 15. The data is cumulated from three experiments. The data was statistically tested using a two-tailed unpaired t-test with Welch’s correction. *p < 0.05, **p < 0.01, ****p < 0.0001 (Supplementary Table 4). The mobile fraction and halftime of recovery of I₂ (B, C) and I₃ (E, F) were extracted from the analysis of the respective curves. Mobile fraction (G) and halftime of recovery (H) of all conditions between I₂-hDLG::GFP and I₃-hDLG::GFP expressing cells were analyzed using a two-tailed unpaired t-test with Welch’s correction. Data are presented as mean values ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 (Supplementary Tables 4 and 5). Source data are provided as Source Data file.