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. 2021 Jul 29;36(8):1631–1638. doi: 10.1038/s41433-021-01706-8

Fig. 5. Effects of HtrA1 overexpression on the growth of ARPE-19 cells.

Fig. 5

a Cell proliferation of ARPE-19 cells transfected with HtrA1 construct EX-M0558-M06 analysed using CCK-8, and the result was recorded using lipo-treated cells at 100%. b Cell morphology of ARPE-19 cells after transfected with the HtrA1 construct EX-M0558-M06. The magnification is ×40. c Flow cytometric evaluation of ARPE-19 apoptosis. Histograms derived from flow cytometry comparing apoptotic cells between vehicle and HtrA1 transfected cells. Twenty-four hours after transfection, the induction of apoptosis was determined using flow cytometric analysis of Annexin V-FITC and PI-stained ARPE-19 cells. Cells in the lower right quadrant indicate Annexin positive, early apoptotic cells, and cells in the upper right quadrant indicate Annexin positive/PI positive, late apoptotic cells. Results were expressed as means ± SD. **p < 0.01 vs. Lipo-treated control. Control, non-transfected ARPE-19 cells. Lipo, performed with Lipofectamine® LTX&PLUS™ reagent. HtrA1+, transfected with HtrA1 construct EX-M0558-M06.