Reagent or resource	Source	Identifier
Antibodies
Purified anti-mouse CD16/32	Biolgend	Cat# 101302;
APC anti-mouse CD4	Biolgend	Cat# 100412
PE anti-mouse CD8	Biolgend	Cat# 100708
Anti-mouse β-Actin (Actin)	Abcam	Cat# ab8227
Pecy5.5 anti-mouse CD45	Biolgend	Cat# 368504
PE anti-mouse NK1.1	ebioscience	Cat# 12-5941-82
anti-mouse CD8α antibody	BioXcell	Cat# BE0061
Anti-goat Lamin B	Santa Cruz	Cat# sc-6216; RRID: AB_648156
Anti-P65	CST	Cat# 4764, RRID:AB_823578
Anti-pP65	CST	Cat# 3033
Anti-TBK1	CST	Cat# 3504, RRID: AB_2255663
Anti-pTBK1	CST	Cat# 5483 S
Anti-cGAS	CST	Cat# 15102
Anti-IRF3	Abcam	Cat# Ab25950
Anti-pIRF3	Abcam	Cat# Ab138449
Anti-rabbit IgG HRP-linked	CST	Cat# 7074; RRID: AB_2099233
Anti-mouse IgG HRP-linked	CST	Cat# 7076; RRID: AB_330924
Complete protease inhibitor cocktail tablets	Roche	Cat#11697498001
Chemicals, peptides, and recombinant proteins
TRIzol™ reagent	ThermoFisher Scientific	Cat# 15596026
Lipofectamine 3000 transfection reagent	ThermoFisher Scientific	Cat# L3000015
Critical commercial assays
Cytofix/cytoperm fixation/ Permeabilization kit	BD Bioscience	Cat# 554714
cell staining buffer	Biolegend	Cat# 420201
Experimental models: cell lines
Human: HEK293T	ATCC	Cat# CRL-3216; RRID: CVCL_0063
Human: U2OS	ATCC	Cat# SCSP-5030
Human: HCT116	ATCC	Cat# TCHu 99
Mouse: MC38	ATCC	Cat# HTX2138
Mouse: MEF	ATCC	Cat# CBP60599
Experimental models: organisms/strains
C57BL/6 mice	Jackson laboratory	Cat# 000664
Recombinant DNA
pCDH-mSENP3-WT	This paper	N/A
pCDH-mSENP3–9A	This paper	N/A
pCDH-SENP3-WT	This paper	N/A
pCDH-SENP3–9A	This paper	N/A
pCDH-SENP3–9E	This paper	N/A
pLVX-TetOne-Puro-SENP3-WT	This paper	N/A
pLVX-TetOne-Puro-SENP3–9A	This paper	N/A
Px459-mcGAS-Puro	This paper	N/A
Px459-SENP3-Puro	This paper	N/A
Software and algorithms
FlowJo v.10.CL	FlowJo	RRID:SCR_008520
Prism v.7.0e	GraphPad	RRID:SCR_002798
Other
BD FACSVerseTM	BD Biosciences	N/A