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Bone microvascular endothelial cell (BMEC) culture and identification. A,B, The typical morphologies of first passage (P1) and second passage (P2) of BMECs (50 x). C, BMEC identification by the marker of CD31. D, BMEC identification by the marker of vWF. E, the ratio of positive cells in CD31 and vWF staining (n = 3, each group consisted of three repeated wells for analysis).
