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. 2022 Jul 21;36:44–51. doi: 10.1016/j.jot.2022.07.001

Figure 3.

Figure 3

Reduced cell viability and increased necroptosis of BMECs after TNF-α stimulation. A, Cell Counting Kit-8 results of cell viability after incubation with TNF-α for 24 ​h (n ​= ​3, each group consisted of three repeated wells for analysis). B, the TUNEL staining results of the control group. C, the TUNEL staining results of the 100 ​ng/ml TNF-α group. D, the percentage of cell necroptosis from the TUNEL staining in the 100 ​ng/ml TNF-α and control group (n ​= ​3, each group consisted of three repeated wells for analysis). E, (1)–(3) the BMECs were stained with the Annexin V-FITC/propidium iodide (PI) in the control, 100 ​ng/ml TNF-α and 100 ​ng/ml TNF-α ​+ ​500 ​nM AptTNF-α group, respectively. The cells in the Q2(UR) and Q1(UL) stand for the percentage of necroptosis. (4) Quantitative data analyses of the Annexin V-FITC/PI results in these three groups (n ​= ​3, each group was repeated three times in six-well plate for analysis). ∗p ​< ​0.05. BMEC, bone microvascular endothelial cell. Apt 50, 50 ​nM TNF-α aptamer. Apt 100, 100 ​nM TNF-α aptamer. Apt 500, 500 ​nM TNF-α aptamer.