Table 1.
Gene | Forward sequence | Reverse sequence |
---|---|---|
RIP1 | 5′-TCCTCGTTGACCGTGAC-3′ | 5 -GCCTCCCTCTGCTTGTT-3′ |
RIP3 | 5′-CCAGCTCGTGCTCCTTGACT-3′ | 5′-TTGCGGTCCTTGTAGGTTTG-3′ |
MLKL | 5′-TCTCCCAACATCCTGCGTAT-3′ | 5′-TCCCGAGTGGTGTAACCTGTA-3′ |
GAPDH | 5′-CGCTAACATCAAATGGGGTG-3′ | 5′-TTGCTGACAATCTTGAGGGAG-3′ |
RIP1, the receptor-interacting protein kinase 1. RIP3, the receptor-interacting protein kinase 3. MLKL, the mixed lineage kinase domain-like protein. GAPDH, the glyceraldehyde-phosphate dehydrogenase.