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. 2022 Jun 2;14(3):587–607. doi: 10.1016/j.jcmgh.2022.05.012

Figure 12.

Figure 12

Sivelestat-mediated NE blockade protects against ALF in mice. (A) Ten-week-old male C57 BL/6J mice were pretreated with sivelestat (SIV, 150 mg kg-1 body weight) via a single intraperitoneal injection 24 hours before ALF induction with intraperitoneal injection of D-GalN (0.75 mg g-1 body weight) and LPS (2.5 μg g-1 body weight). (B) Histologic lesions in the liver were assessed by H&E staining as shown in representative images (original magnification, 400×). Scale bar: 10 μm. (C) Quantification of necrotic area in H&E-stained liver sections. Serum activity of (D) ALT and (E) AST was measured to evaluate hepatocellular damage. (F) mRNA expression level of TNF-α in the liver tissue determined by quantitative PCR. (G) Representative images of immunohistochemistry of MPO in liver section (upper; scale bar: 10 μm) and semiquantification of MPO+ cells in each 40× microscopic field (lower). (H) Immunoblotting analysis of MPO in liver lysate (upper) and densitometric analysis of MPO protein abundance relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control (lower). Data are expressed as means ± SEM. n = 5–6. ∗P < .05, ∗∗P < .01.