Figure 13.

Pharmacologic inhibition of NE protects against ALF in miR-223 KO mice and WT control mice. (A) The pretreatment with sivelestat (SIV) was conducted in 10-week-old male miR-223 KO mice and WT control mice via a single intraperitoneal injection of D-GalN (0.75 mg g^-1 body weight) and LPS (2.5 μg g^-1 body weight) 24 hours before ALF induction. (B) Histologic lesions were assessed by H&E staining in liver sections as shown in representative images (original magnification, 400×) Scale bar: 10 μm. (C) Quantification data of necrotic area in H&E-stained liver sections. Serum levels of (D) ALT and (E) AST was measured to evaluate hepatocellular damage. (F) TNF-α mRNA levels in liver tissue were determined by quantitative PCR. Data are expressed as means ± SEM. n = 5–6. ∗P < .05, ∗∗P < .01.