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. 2022 Jun 2;14(3):587–607. doi: 10.1016/j.jcmgh.2022.05.012

Figure 2.

Figure 2

LPS treatment induces slight hepatocellular injury and mild NET formation in liver in miR-223 KO mice and WT controls. (A) Ten-week-old male miR-223 KO mice with C57 BL/6J genetic background and WT control mice were injected intraperitoneally with LPS (2.5 μg g-1 body weight) dissolved in PBS as vehicle to induce ALF. (B) Representative images of H&E-stained liver sections. Scale bar: 10 μm. (C) Hepatocellular damage as determined by measured serum activity of ALT (left) and AST (right). (D) Hepatic influx of neutrophils was determined by immunoblotting analysis of MPO in liver lysate. (E) Immunohistochemistry of MPO in liver tissues (scale bar: 10 μm) and (F) semiquantification of MPO-positive cells. (G) Immunoblotting analysis of H3Cit in liver lysate from miR-223 KO mice and WT controls with treatment of either LPS or PBS (upper), and densitometric analysis of H3Cit protein abundance relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control (lower). Liver samples from WT mice with D-GalN/LPS–induced ALF serve as positive controls. (H) NETs were detected by immunofluorescence of H3Cit, MPO, and DAPI in liver sections from miR-223 KO mice and WT controls with treatment of either LPS or PBS. Scale bar: 5 μm. Data are expressed as means ± SEM. n = 5–6. ∗P < .05, ∗∗P < .01.