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. 2022 Jun 2;14(3):587–607. doi: 10.1016/j.jcmgh.2022.05.012

Figure 9.

Figure 9

ALF results in the formation of NETs in the liver under regulation of NE. (A) Immunofluorescence of H3Cit (red), NE (green), and DAPI (blue) in liver sections showing NE as a component of NETs in mouse liver with ALF (scale bar: 5 μm). (B) The formation of NETs was detected by immunofluorescence of H3Cit (red), NE (green), and DAPI (blue) in mouse primary neutrophils with ex vivo stimulation by LPS (200 μg mL-1) for 3 hours (scale bar: 1 μm). Immunofluorescence of H3Cit (red), MPO (green), and DAPI (blue) in primary neutrophils isolated from NE KO mice and WT control mice with ex vivo stimulation by LPS (C, scale bar: 5 μm) to identify the formation of NETs and (D) semiquantification under 40× microscopic fields. Representative images of immunofluorescence of H3Cit, MPO, and DAPI in liver sections from 10-week-old male NE KO mice and WT control mice treatment with (E, scale bar: 5 μm) D-GalN/LPS or PBS as vehicle and (F) semiquantification of the number of NETs under 40× microscopic fields, indicating the impaired capacity for NET formation in NE KO mice under ALF conditions. Ex vivo data were analyzed according to 3 independent experiments. Data in animal studies are expressed as means ± SEM. n = 5. ∗∗P < .01.