Table 2.
Key Resources
| Antibodies and reagents | Source | Identifier |
|---|---|---|
| Goat anti-myeloperoxidase | R&D Systems (Minneapolis, MN) | Cat# AF3667; RRID: AB_2250866 (0.5 μg/mL in WB; 5 μg/mL in IHC; 10 μg/mL in IF) |
| Rabbit anti–histone H3 | Abcam (Cambridge, UK) | Cat# ab5103; RRID: AB_304752 (1 μg/mL in WB; 10 μg/mL in IF) |
| Rat anti–neutrophil elastase | R&D Systems (Minneapolis, MN) | Cat# MAB4517 (0.5 μg/mL in WB; 5 μg/mL in IHC; 10 μg/mL in IF) |
| Rabbit anti-HSP90 | Cell Signaling Technology (Boston, MA) | Cat# 4877S; RRID: AB_2233307 (1:1000 dilution in WB) |
| Rabbit anti-GAPDH | Cell Signaling Technology (Boston, MA) | Cat# 2118S; RRID: AB_561053 (1:1000 dilution in WB) |
| Rabbit anti-F4/80 | Cell Signaling Technology (Boston, MA) | Cat# 70076S; RRID: AB_2799771 (1:300 dilution in IHC) |
| Donkey anti-goat secondary Ab | R&D Systems (Minneapolis, MN) | Cat# HAF109; RRID: AB_357236 (1:1000 dilution in WB) |
| Goat anti-rabbit secondary Ab | Cell Signaling Technology (Boston, MA) | Cat# 7074S; RRID: AB_2099233 (1:2000 dilution in WB) |
| Goat anti-rat secondary Ab | Cell Signaling Technology (Boston, MA) | Cat# 7077S; RRID: AB_10694715 (1:2000 dilution in WB; 1:500 dilution in IHC) |
| Rabbit anti-goat secondary Ab | Thermo Fisher Scientific (Waltham, MA) | Cat# 31402; RRID: AB_228395 (1.5 μg/mL in IHC) |
| Donkey anti-rabbit Alexa Fluor 568 | Thermo Fisher Scientific (Waltham, MA) | Cat# A10042; RRID: AB_2534017 (5 μg/mL in IF) |
| Chicken anti-rat Alexa Fluor 488 | Thermo Fisher Scientific (Waltham, MA) | Cat# A21470; RRID: AB_2535873 (5 μg/mL in IF) |
| Chicken anti-goat Alexa Fluor 488 | Thermo Fisher Scientific (Waltham, MA) | Cat# A21467; RRID: AB_2535870 (5 μg/mL in IF) |
| DAPI | Thermo Fisher Scientific (Waltham, MA) | Cat# D1306; RRID: AB_2629482 |
Ab, antibody; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HSP90, heat shock protein 90; IF, immunofluorescence; IHC, immunohistochemistry; RRID, Research Resource Identifier; WB, Western blot analysis.