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. 2022 Jun 15;14(3):567–586. doi: 10.1016/j.jcmgh.2022.06.002

Figure 9.

Figure 9

Genetic ablation of Tlr9 reduces H felis-induced gastric inflammation. (A–D) Representative photomicrographs showing (A) H&E-stained, (B) CD45-stained, (C) B220-stained, and (D) CD3-stained cross sections under low power (left images) and high power (right images) magnification through the body of stomachs from Tlr9-/- and WT mice at 4 months after oral gavage with either broth or H felis. Also shown are the corresponding graphs depicting (A) the inflammation scores or (B–D) positive cells/high power field (HPF). Scale bars: 200 μm (left images) and 50 μm (right images). n = 6 mice/group. ∗P < .05, ∗∗P < .01, ∗∗∗∗P < .0001; one-way ANOVA. (E) qPCR expression of representative inflammatory genes (relative to 18s rRNA) in gastric corpus (body) tissues from the stomachs of WT and Tlr9-/- mice at 4 months after oral gavage with either broth or H felis. n = 6 mice/group. ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001; one-way ANOVA. (F and G) In (F), qPCR expression of H felis flaB (relative to Gapdh), and (G) representative agarose gel of reverse transcription PCR of H felis flaB along with Gapdh, in stomachs from WT and Tlr9-/- mice at 4 months after oral gavage with either broth or H felis. n = 6 mice/group. ∗∗P < .01, ∗∗∗∗P < .0001; one-way ANOVA.