TABLE 2.
RNA-Seq HLA typing results utilizing NGSEngine
| Sample ID | HLA-A | HLA-B | HLA-C | HLA-DPA1 | HLA-DPB1 | HLA-DQA1 | HLA-DQB1 | HLA-DRB1 | HLA-DRB3/4/5 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Reference | NGSEngine | Reference | NGSEngine | Reference | NGSEngine | Reference | NGSEngine | Reference | NGSEngine | Reference | NGSEngine | Reference | NGSEngine | Reference | NGSEngine | Reference | NGSEngine | |
| cDNA001 | 02:01:01 | 02:01:01 | 35:01:01 | 35:01:01 | 03:04:01 | 03:04:01 | 01:03:01 | 01:03:01 | 02:01:02 | 02:01:02 | 01:01:01 | 01:01:01 | 05:01:01 | 05:01:01 | 01:01:01 | 01:01:01 | ||
| 11:01:01 | 11:01:01 | 40:01:02 | 40:01:02 | 04:01:01 | 04:01:01 | - | - | 03:01:01 | 03:01:01 | 01:04:01 | 01:04:01 | 05:03:01 | 05:03:01 | 14:54:01 | 14:54:01 | 3*02:02:01 | 3*02:02:01 | |
| cDNA057 | 02:01:01 | 02:01:01 | 07:06:01 | 07:06:01 | 15:05:02 | 15:05:02 | 01:03:01 | 01:03:01 | 04:01:01 | 04:01:01 | 02:01:01 | 02:01:01 | 02:02:01 | 02:02:01 | 11:02:01 | 11:02:01 | 3*02:02:01 | 3*02:02:01 |
| 03:01:01 | 03:01:01 | 44:03:01 | 44:03:01 | 16:01:01 | 16:01:01 | - | - | - | - | 05:05:01 | 01:01:01 | 03:19:01 | 03:19:01 | 07:01:01 | 07:01:01 | 4*01:01:01 | 4*01:01:01 | |
| cDNA058 | 03:01:01 | 03:01:01 | 53:01:01 | 53:01:01 | 04:01:01 | 04:01:01 | 01:03:01 | 01:03:01 | 04:01:01 | 04:01:01 | 03:03:01 | 03:03:01 | 02:02:01 | 02:02:01 | 11:01:02 | 11:01:02 | 3*02:02:01 | 3*02:02:01 |
| - | - | 57:02:01 | 57:02:01 | 18:02:01 | 18:02:01 | 03:01:01 | 03:01:01 | 105:01:01 | 105:01:01 | 05:05:01 | 05:05:01 | 03:19:01 | 03:19:01 | - | - | |||
| cDNA061 | 11:01:01 | 11:01:01 | 40:01:02 | 40:01:02 | 03:03:01 | 03:03:01 | 01:03:01 | 01:03:01 | 04:01P | 04:01:01 | 03:01:01 | 03:01:01 | 03:01:01 | 03:01:01 | 11:01:01 | 11:01:01 | 3*02:02:01 | 3*02:02:01 |
| 24:02:01 | 24:02:01 | 55:01:01 | 55:01:01 | 03:04:01 | 03:04:01 | 02:01:01 | 02:01:01 | 10:01P | 10:01:01 | 05:05:01 | 05:05:01 | 03:02:01 | 03:02:01 | 04:04:01 | 04:04:01 | 4*01:03:01 | 4*01:03:01 | |
| cDNA062 | 30:01:01 | 30:01:01 | 35:01:01 | 35:01:01 | 04:01:01 | 04:01:01 | 01:03:01 | 01:03:01 | 04:01P | 04:01:01 | 01:02:01 | 01:02:01 | 03:19:01 | 03:19:01 | 11:02:01 | 11:02:01 | 3*02:02:01 | 3*02:02:01 |
| 30:02:01 | 30:02:01 | 42:01:01 | 42:01:01 | 17:01:01 | 17:01:01 | - | - | 03:01P | 104:01:01 | 05:05:01 | 05:05:01 | 06:09:01 | 06:09:01 | 13:02:01 | 13:02:01 | 3*03:01:01 | 3*03:01:01 | |
| cDNA068 | 02:01:01 | 02:01:01 | 07:02:01 | 07:02:01 | 07:02:01 | 07:02:01 | 01:03:01 | 01:03:01 | 02:01:02 | 02:01:02 | 02:01:01 | 02:01:01 | 02:02:01 | 02:02:01 | 04:01:01 | 04:01:01 | 4*01:01:01 | 4*01:01:01 |
| 03:01:01 | 03:01:01 | 44:03:01 | 44:03:01 | 16:01:01 | 16:01:01 | 02:01:01 | 02:01:01 | 11:01:01 | 11:01:01 | 03:01:01 | 03:01:01 | 03:02:01 | 03:02:01 | 07:01:01 | 07:01:01 | 4*01:03:01 | 4*01:03:01 | |
| cDNA069 | 30:01:01 | 30:01:01 | 42:01:01 | 42:01:01 | 06:02:01 | 06:02:01 | 02:01:01 | 02:01:01 | 01:01P | 01:01:01 | 01:05:01 | 01:05:01 | 04:02:01 | 04:02:01 | 03:02:01 | 03:02:01 | 3*01:62:01 | 3*01:62:01 |
| 68:01:01 | 68:01:01 | 58:02:01 | 58:02:01 | 17:01:01 | 17:01:01 | 02:02:02 | 02:02:02 | 11:01P | 11:01:01 | 04:01:01 | 04:01:01 | 05:01:01 | 05:01:01 | 12:01:01 | 12:01:01 | 3*02:02:01 | 3*02:02:01 | |
| cDNA070 | 23:01:01 | 23:01:01 | 44:03:01 | 44:03:01 | 04:01:01 | 04:01:01 | 01:03:01 | 01:03:01 | 17:01:01 | 17:01:01 | 01:02:01 | 01:02:01 | 05:02:01 | 05:02:01 | 13:02:01 | 13:02:01 | 3*03:01:01 | 3*03:01:01 |
| 26:01:01 | 26:01:01 | - | - | - | - | 02:01:01 | 02:01:01 | 18:01:01 | 18:01:01 | - | - | 06:09:01 | 06:09:01 | 13:31 | 13:31 | |||
| cDNA071 | 01:01:01 | 01:01:01 | 08:01:01 | 08:01:01 | 07:01:01 | 07:01:01 | 01:03:01 | 01:03:01 | 01:01:01 | 01:01:01 | 05:01:01 | 05:01:01 | 02:01:01 | 02:01:01 | 03:01:01 | 03:01:01 | 3*01:01:02 | 3*01:01:02 |
| 30:02:01 | 30:02:01 | 47:03 | 47:03 | 07:18:01 | 07:18:01 | 02:02:02 | 02:02:02 | 06:01:01 | 06:01:01 | - | - | - | - | - | - | 3*02:02:01 | 3*02:02:01 | |
| cDNA072 | 03:01:01 | 03:01:01 | 07:02:01 | 07:02:01 | 04:01:01 | 04:01:01 | 02:02:02 | 02:02:02 | 01:01:01 | 01:01:01 | 01:02:01 | 01:02:01 | 03:19:01 | 03:19:01 | 15:03:01 | 15:03:01 | 5*01:01:01 | 5*01:01:01 |
| 23:01:01 | 23:01:01 | 44:03:01 | 44:03:01 | 07:02:01 | 07:02:01 | 03:01:01 | 03:01:01 | 105:01:01 | 105:01:01 | 05:05:01 | 05:05:01 | 06:02:01 | 06:02:01 | 11:02:01 | 11:02:01 | 3*02:02:01 | 3*02:02:01 | |
| cDNA073 | 03:01:01 | 03:01:01 | 35:01:01 | 35:01:01 | 04:01:01 | 04:01:01 | 01:03:01 | 01:03:01 | 17:01:01 | 17:01:01 | 01:02:01 | 01:02:01 | 02:02:01 | 02:02:01 | 07:01:01 | 07:01:01 | 4*01:03:01 | 4*01:03:01 |
| 30:01:01 | 30:01:01 | 44:03:01 | 44:03:01 | - | - | 02:01:01 | 02:01:01 | 18:01:01 | 18:01:01 | 02:01:01 | 02:01:01 | 06:02:01 | 06:02:01 | 15:03:01 | 15:03:01 | 5*01:01:01 | 5*01:01:01 | |
| cDNA074 | 23:01:01 | 23:01:01 | 44:03:01 | 44:03:01 | 04:01:01 | 04:01:01 | 01:03:01 | 01:03:01 | 02:01:02 | 02:01:02 | 01:02:01 | 01:02:01 | 02:02:01 | 02:02:01 | 11:01:02 | 11:01:02 | 3*02:02:01 | 3*02:02:01 |
| 36:01 | 36:01 | 53:01:01 | 53:01:01 | - | - | - | - | - | - | 02:01:01 | 02:01:01 | 06:02:01 | 06:02:01 | 07:01:01 | 07:01:01 | 4*01:01:01 | 4*01:01:01 | |
| cDNA075 | 23:01:01 | 23:01:01 | 45:01:01 | 45:01:01 | 06:02:01 | 06:02:01 | 01:03:01 | 01:03:01 | 01:01:01 | 01:01:01 | 01:02:01 | 01:02:01 | 02:01:01 | 02:01:01 | 03:01 | 03:01:01 | 3*02:02:01 | 3*02:02:01 |
| 23:17 | 23:17 | - | - | 16:01:01 | 16:01:01 | 02:01:08 | 02:01:08 | 02:01:19 | 02:01:19 | 05:01:01 | 05:01:01 | 05:02:01 | 05:02:01 | 11:01 | 11:01:02 | 3*03:01:01 | 3*03:01:01 | |
| cDNA076 | 68:02:01 | 68:02:01 | 15:03:01 | 15:03:01 | 02:10:01 | 02:10:01 | 02:01:01 | 02:01:01 | 05:01:01 | 05:01:01 | 01:01:02 | 01:01:02 | 02:02:01 | 02:02:01 | 07:01:01 | 07:01:01 | 4*01:01:01 | 4*01:01:01 |
| 74:01:01 | 74:01:01 | 15:10:01 | 15:10:01 | 03:04:02 | 03:04:02 | 02:02:02 | 02:02:02 | 11:01:01 | 11:01:01 | 03:03:01 | 03:03:01 | 05:01:01 | 05:01:01 | 01:02:01 | 01:02:01 | |||
| cDNA077 | 02:01:01 | 02:01:01 | 49:01:01 | 49:01:01 | 07:01:02 | 07:01:02 | 01:03:01 | 01:03:01 | 04:02P | 105:01:01 | 01:01:01 | 01:01:01 | 03:19:01 | 03:19:01 | 15:02P | 15:02:01 | 5*01:08:01N | |
| 03:01:01 | 03:01:01 | 51:01:01 | 51:01:01 | 14:02:01 | 14:02:01 | 03:01:01 | 03:01:01 | - | - | 04:01:02 | 04:01:02 | 05:01:24 | 05:01:24 | 08:04:01 | 08:04:01 | |||
| cDNA078 | 02:01:01 | 02:01:01 | 42:01:01 | 42:01:01 | 05:01:01 | 05:01:01 | 01:03:01 | 01:03:01 | 04:02:01 | 04:02:01 | 01:02:01 | 01:02:01 | 03:01:01 | 03:01:01 | 04:01:01 | 04:01:01 | 4*01:03:01 | 4*01:03:01 |
| 30:01:01 | 30:01:01 | 44:02:01 | 44:02:01 | 17:01:01 | 17:01:01 | - | - | 18:01:01 | 18:01:01 | 03:03:01 | 03:03:01 | 06:02:01 | 06:02:01 | 15:03:01 | 15:03:01 | |||
| cDNA079 | 01:01:01 | 01:01:01 | 37:01:01 | 37:01:01 | 03:04:01 | 03:04:01 | 01:03:01 | 01:03:01 | 01:01:01 | 01:01:01 | 01:02:01 | 01:02:01 | 05:01:01 | 05:01:01 | 10:01:01 | 10:01:01 | ||
| 02:01:01 | 02:01:01 | 40:01:02 | 40:01:02 | 06:02:01 | 06:02:01 | 02:01:02 | 02:01:02 | 04:01:01 | 04:01:01 | 01:05:01 | 01:05:01 | 06:02:01 | 06:02:01 | 15:01:01 | 15:01:01 | 5*01:01:01 | 5*01:01:01 | |
| cDNA082 | 11:01:01 | 11:01:01 | 15:10:01 | 15:10:01 | 01:02:01 | 01:02:01 | 01:03:01 | 01:03:01 | 04:01:01 | 04:01:01 | 01:01:01 | 01:01:01 | 05:01:01 | 05:01:01 | 01:01:01 | 01:01:01 | ||
| 33:03:01 | 33:03:01 | 56:01:01 | 56:01:01 | 03:02:02 | 03:02:02 | - | - | 18:01:01 | 18:01:01 | 01:02:01 | 01:02:01 | 06:02:01 | 06:02:01 | 15:03:01 | 15:03:01 | 5*01:01:01 | 5*01:01:01 | |