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CB1 mRNA expression in glutamate neurons in the ventral tegmental area (VTA) by RNAscope in situ hybridization (ISH) assays. CB1R mRNA (green, arrows) was co-localized with VgluT2 mRNA (red) in glutamate neurons (red, arrows) in VgluT2-Cre mice A, but not in glutamate-CB1-KO mice B. CB1 mRNA was still detectable in other non-glutamate (VgluT2-negative, open arrows) neurons in the VTA in glutamate-CB1-KO mice B
