Table 2.
Potential contribution of demographic and behavioral co-variates to epigenetic measures over time, using mixed effects models
| Potential Contributors to Epigenetic Measures | F value (p value)a |
||||
|---|---|---|---|---|---|
| AAR | EEAA | PEAA | GEAA | aaDNAmTL | |
| Study Visit, Visit A vs B | 11.00 (0.001) | 38.85 (<0.001) | 21.01 (<0.001) | 1.56 (0.21) | 75.13 (<0.001) |
| HIV Serostatus Group, SC vs SNb | 1.99 (0.16) | 5.29 (0.02) | 10.22 (0.002) | 3.39 (0.07) | 15.14 (<0.001) |
| Study Visit∗HIV Serostatus Group | 1.66 (0.20) | 29.68 (<0.001) | 26.23 (<0.001) | 0.00 (0.96) | 63.44 (<0.001) |
| Race, non-white vs white | 0.36 (0.55) | 1.70 (0.19) | 3.37 (0.07) | 30.38 (<0.001) | 4.52 (0.03) |
| Hepatitis B Status, HBsAg – vs + c | 1.22 (0.27) | 0.19 (0.66) | 0.14 (0.71) | 0.10 (0.75) | 0.70 (0.40) |
| BMI, kg/m2 | 0.02 (0.89) | 0.17 (0.68) | 0.10 (0.75) | 0.79 (0.38) | 0.15 (0.70) |
| Smoking, cumulative pack years | 0.01 (0.93 | 0.03 (0.87) | 1.79 (0.18) | 47.15 (<0.001) | 1.5 (0.22) |
AAR = Age-Acceleration Residual, EEAA = Extrinsic Epigenetic Age Acceleration, PEAA = Phenotypic Epigenetic Age Acceleration, aaDNAmTL = age-adjusted DNA methylation-based estimate of Telomere Length, HBsAg = Hepatitis B surface Antigen, BMI = Body Mass Index.
F values and Pr > F p values (p values in italics, bold if < 0.05) from mixed models incorporating all potential co-variates for all participants at both visits (n = 387 out of 407 total observations due to missing data for some co-variates) in a single model (see also Table S2).
HIV serostatus groups classified as SC (became HIV-infected and seroconverted between Visits A and B) vs SN (persistently HIV-uninfected and seronegative at Visits A and B).
Hepatitis B virus status classified by current HBsAg at visit, negative vs positive.