REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD3-PerCP	BD Biosciences	SK7 (RUO (GMP)), Cat#347344; RRID: AB_400286
CD4-V450	BD Biosciences	RPA-T4 (RUO), Cat#561838; RRID: AB_10924599
CD8-APC-Cy7	BD Biosciences	SK1 (RUO (GMP)), Cat#348793; RRID: AB_400383
CD45RA-PE-Cy7	BD Biosciences	L48 (RUO (GMP)), Cat#337167; RRID: AB_647424
CCR7-AF647	BD Biosciences	150,503 (RUO), Cat#560816; RRID: AB_2033948
HLA DR-BV605	BD Biosciences	G46-6 (RUO), Cat#562845; RRID: AB_2744478
CD38-PE	BD Biosciences	HB7 (RUO (GMP)), Cat#347687; RRID: AB_400341
CD28-PE	BD Biosciences	L293 (RUO (GMP)), Cat#348047; RRID: AB_400368
CD57-BV605	Biolegend	QA17A04 (RUO), Cat#393304; RRID: AB_2728426
CD4-BV510	Biolegend	OKT4 (RUO), Cat#317444; RRID: AB_2561866
IgG2a-AF647	BD Biosciences	G155-178 (RUO), Cat#557715; RRID: AB_396824
IgG2a-BV605	BD Biosciences	G155-178 (RUO), Cat#562778; RRID: AB_2869434
IgG1-PE	BD Biosciences	×40 (RUO (GMP)), Cat#349043; RRID: AB_400398
IgG1-PE-Cy7	BD Biosciences	MOPC-21 (RUO), Cat#557872; RRID: AB_396914
Biological samples
Viably-frozen peripheral blood mononuclear cells	MACS/WIHS Combined Cohort Study (MWCCS)	MWCCS concept sheet number C15039
Chemicals, peptides, and recombinant proteins
Zombie Aqua Fixable Viability Kit	Biolegend	(RUO), Cat#423102
Critical commercial assays
DNeasy Blood & Tissue Kit	QIAGEN	Cat#69506
Quant-it PicoGreen dsDNA Assay Kit	Invitrogen	Cat#P7589
EZ-96 DNA Methylation Kit	Zymo Research	Cat#D5004
Infinium MethylationEPIC BeadChip	Illumina	Cat#WG-317-1003
Deposited data
Raw methylation data	This paper	MACS/WIHS Combined Cohort Study (MWCCS) when study aims are completed per MWCCS policy, via the concept sheet approval process (https://statepi.jhsph.edu/mwccs/work-with-us/); MWCCS concept sheet number C15039
Calculated age-regressed epigenetic clock and estimated telomere length data, as well as necessary de-identified demographic or descriptive data	This paper	MWCCS upon reasonable request via the concept sheet approval process (https://statepi.jhsph.edu/mwccs/work-with-us/); MWCCS concept sheet number C15039
Software and algorithms
Epigenetic clock software Cell proportion imputation software	http://dnamage.genetics.ucla.edu	Horvath (2013); Hannum et al., 2013; Chen et al., 2016; Levine et al., 2018; Lu, Quach, et al., 2019a; Lu, Seeboth, et al., 2019b; Horvath and Levine (2015); Horvath et al., 2016
Weighted Gene Correlation Network Analysis, WGCNA R package	This paper	Langfelder and Horvath (2008)
EnrichR gene list enhancement tool	This paper	Kuleshov et al. (2016)
Attune NxT Software	ThermoFisher	Cat#A25556