Figure 3.

Off-target binders can be identified via cross-panning with unrelated targets

(A and B) Overview of cross-panning experiments against BV extract and BSA.

(B) Overlap of specific sequences identified by R4 on target and cross-panning against unrelated targets. Black circles connected by lines indicate a set made up of sequences denoted by left text labels, and bars reflect the number of members in that set.

(C) Amino acid enrichment over 10 internal CDR-H3 positions (colored bars) for sequences identified to be polyspecific via panning against unrelated targets.

(D) Amino acid enrichment over 10 internal CDR-H3 positions (colored bars) for sequences identified to be nonspecific via cross-panning experiments with Herceptin/Xolair.

(E) Top two enriched motifs of sequences specific to trastuzumab/omalizumab, nonspecific sequences identified by cross-panning against trastuzumab/omalizumab, and polyspecific sequences identified by panning against BSA, BV extract, and TGF-β.