Fig. 4.

Mmu_circ_0001052 in exosomes promoted proliferation, migration and angiogenesis of high glucose-induced HUVECs via miR-106a-5p and FGF4/p38MAPK pathway. A the target of miR-106a-5p and mmu_circ_0001052 via starbase3.0. B Dual-luciferase reporter gene assay. C RNA binding protein immunoprecipitation. D The expression levels of miR-106a-5p, mmu_circ_0001052 and FGF4 via real-time PCR. E proliferation by MTT. F migration by transwell. G, angiogenesis via tube formation assay. H the level of FGF4/p38MAPK pathway via western blot. All data were obtained from at least three replicate experiments. *P < 0.05, **P < 0.01. N = 3. One-way ANOVA was used to analyze the differences among groups. Afterward, post-pairwise comparison was conducted by Dunnett’s multiple comparison test. Comparison among multiple groups at different time points was analyzed by repeated measures ANOVA