Fig. 7.

Overexpression of BTG2 aggravated OGD-induced injury damage and reversed the protective effect of miR-21. A Western blot analyzed Bax, Bcl2, and cleaved-caspase-3 protein levels in hypoxic and ischemic H9c2 cells treated with PBS, vector and BTG2. Relative protein levels were presented as the average expression normalized to GAPDH (n = 3) and TUNEL analysis (n = 3) B Green, TUNEL-positive nuclei; blue, DAPI-stained nuclei. Scale bars = 50 μm. And flow cytometric analysis (n = 3) (C). D H9c2 cells treated with control, OGD, OGD  + miR-21 mimics and OGD  + miR-21 mimics + BTG2. Relative Bax, Bcl2, and cleaved-caspase-3 protein levels among different groups were presented as the average expression normalized to β-Tubulin (n = 3). Data were presented as mean ± SEM. Statistical analysis was performed with one-way ANOVA followed by Bonferroni’s correction. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001