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. 2022 Jul 22;38:23. doi: 10.1186/s42826-022-00133-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Top 30 differentially expressed genes. Increased tidal volume leads to increased differential expression. Shown are the top 30 genes with the highest beta value from RNA sequencing of mouse lung tissues. Up regulated genes showed increased expression with increased tidal volume, while down regulated genes decreased in expression with increased tidal volume (A). Genes involved in glutathione production were significantly upregulated. The expression was shown to be tidal volume dependent (B). Real time PCR was done using lung tissue from ventilated mice and compared to SB controls (C). The top three genes from sequencing analysis were confirmed to be expressed in a tidal volume dependent manner. Immunoblotting from SB control, 10, 20 and 30 mL/kg tidal volume ventilated mice was performed for Keap1 and Actin. Actin used as a loading control and quantification carried out using FIJI (D). Significant difference from the SB controls are shown (P ≤ 0.05 = *; P ≤ 0.01 = **; P ≤ 0.001 = ***)