Fig. 3.

A 1.5% agarose gel obtained from the amplification of partial fragments of sand fly 18srDNA and Leishmania ITS1 genes with the multiplex PCR assay using different concentrations of 18SrRDNA sand fly primers (1–4: 1, 0.1, 0.01 and 0.001 µM) and 1 µM of ITS1 primers