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Primer sequences and corresponding target genes used in the study
| Primer name | DNA sequences | Target gene /PCR product size | References |
|---|---|---|---|
| ITS1219NGSF | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAGCTGGATCATTTTCCGATG | Leishmania ITS1/343 bp | [33] |
| ITS1219NGSR | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGATCGCGACACGTTATGTGAG | ||
| SFNGSF | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTGCGGTTAAAACGTTCGTAG | Sand flies 18S rDNA/230 bp | [44] |
| SFNGSR | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGACCGGTAAAACATCCGTCAC | This study |
