Figure 10.

Behavior analyses of wfikkn1 mutant zebrafish exposed to 50 pg/ml 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). A, 120-h postfertilization larval photomotor response (LPR) showing wfikkn1 mutant zebrafish significantly hypoactive compared with wild-type (WT) zebrafish in light (quiescent) and dark (active) periods of the assay indicated by the gray-black bar at the bottom, respectively. Significant interaction between lack of wfikkn1 and TCDD exposure was detected in Cycle 2. Error bars are a representation of the standard deviation of the mean every 6 seconds. B, 28-dpf mirror assay showing mutant zebrafish spending significantly less time in the “mirror zone” compared with WT zebrafish. Boxplots (for B–D) show spread of the data, with the horizontal line representing each median, and dots representing outliers. 28-dpf juvenile (C) and adult (D) shoaling measured using the nearest neighbor distance (NND), interindividual distance, and speed of zebrafish within shoals of 4 zebrafish. NND was significantly different between mutants and WT in juvenile zebrafish, and all 3 endpoints were significant in adult zebrafish. E, Summary table showing assay details, including p values and corresponding significance (Type III ANOVA).