FIGURE 5.

Triple confocal microscopic images representing in‐vitro CBR1 and CBR2 (green) expression in equine primary skin‐derived cells. Immunoreactivity in the primary skin‐derived keratinocytes was detected for CBR1 (A‐D) and CBR2 (I‐L). Similarly, primary skin‐derived dermal cells were, in most present fibroblasts, also positive for CBR1 (E‐H) as well as CBR2 (M‐P). The cell cytoskeletal analysis of F‐actin (Phalloidin‐AF555 staining, red), with its content and distribution, was performed to distinguish keratinocytes (B and J) from fibroblasts (F and N). The DAPI (blue) was used for nucleus counterstaining of keratinocytes (C and K) and fibroblasts (G and O). Images were taken under 40x magnification; scale bars = 20 μm