| Antibodies |
| Mouse monoclonal anti-Acetylated tubulin |
MilliporeSigma |
Cat# T6793; RRID:AB_477585 |
| Mouse monoclonal anti-ACTA2 |
Thermo Scientific Lab Vision |
Cat# MS-113-P0; RRID:AB_64001 |
| Goat polyclonal anti-CC10 (S-20) |
Santa Cruz Biotechnology |
Cat# sc-9773; RRID:AB_2183391 |
| Chicken polyclonal anti-GFP |
Aves Labs |
Cat# GFP-1010; RRID:AB_2307313 |
| Mouse monoclonal anti-HOP (E-1) |
Santa Cruz Biotechnology |
Cat# sc-398703; RRID:AB_2687966 |
| Mouse monoclonal anti-Neurofilament (NF-M) |
Developmental Studies Hybridoma Bank |
Cat# 2H3; RRID:AB_531793 |
| Rabbit monoclonal anti-NKX2.1 |
Epitomics |
Cat# 2044-1; RRID:AB_1267367 |
| Rabbit polyclonal anti-PDGF receptor alpha |
Cell Signaling Technology |
Cat# 3164; RRID:AB_2162351 |
| Rabbit monoclonal anti-Phospho-PDGF Receptor α (Tyr754) (23B2) |
Cell Signaling Technology |
Cat# 2992; RRID:AB_390728 |
| Goat polyclonal anti-PDGF R beta |
R&D Systems |
Cat# AF1042; RRID:AB_2162633 |
| Rat monoclonal anti-PECAM-1 (MEC 13.3) (CD31) |
Santa Cruz Biotechnology |
Cat# sc-18916; RRID:AB_627028 |
| Rabbit polyclonal anti-PGP9.5 |
AnaSpec |
Cat# 53772; RRID:AB_2272470 |
| Rabbit polyclonal anti-prosurfactant protein C (proSP-C) |
MilliporeSigma |
Cat# AB3786; RRID:AB_91588 |
| Mouse monoclonal anti-SV2 |
Developmental StudiesHybridoma Bank |
Cat# SV2; RRID:AB_2315387 |
| Rabbit monoclonal anti-Synaptophysin (SYP) |
Invitrogen |
Cat# 180130; RRID:AB_10836766 |
| Hamster monoclonal anti-T1α |
Developmental Studies Hybridoma Bank |
Cat# 8.1.1; RRID:AB_531893 |
| Mouse monoclonal anti-TUBB3 |
Developmental Studies Hybridoma Bank |
Cat# 6G7; RRID:AB_528497 |
| Rabbit polyclonal anti-Tyrosine hydroxylase |
Synaptic Systems |
Cat# 213 102; RRID:AB_2619896 |
| Rabbit polyclonal anti-VAChT |
Synaptic Systems |
Cat# 139 103; RRID:AB_887864 |
| Rat monoclonal anti-VANGL2 clone 2G4 |
MilliporeSigma |
Cat# MABN750; RRID:AB_2721170 |
| Bacterial and virus strains |
|
|
|
| Biological samples |
|
|
| Human emphysema/COPD patient tissues |
Paul Wolters, University of California, San Francisco |
N/A |
| Chemicals, peptides, and recombinant proteins |
| 5-Bromo-4-Chloro-3-Indolyl-D-Galactoside (X-gal) |
Research Products International |
Cat# B71800-1.0 |
| Collagenase B |
Roche |
Cat# 11088815001 |
| 2’-Deoxy-5-ethynyluridine (EdU) |
BiosynthCarbosynth |
Cat# 61135-33-9 |
| Deoxyribonuclease I from bovine pancreas |
MilliporeSigma |
Cat# 9003-98-9 |
| Dispase |
BD Biosciences |
Cat# 354235 |
| DMEM |
Mediatech |
Cat# 10-013-CV |
| Fetal Bovine Serum (FBS) |
Gibco |
Cat# 10437-028 |
| Fibronectin |
Corning |
Cat# 354008 |
| Glutaraldehyde, 8% aqueous solution, EM grade |
Electron Microscopy Sciences |
Cat# 16000 |
| 4-Hydroxytamoxifen (4-OHT) |
Toronto Research Chemicals |
Cat# H954705 |
| Nerve Growth Factor (NGF 2.5S) |
Gibco |
Cat# 13257-019 |
| Paraformaldehyde |
MilliporeSigma |
Cat# P6148 |
| Paraformaldehyde, 16% solution, EM grade |
Electron Microscopy Sciences |
Cat# 15700 |
| Penicillin/Streptomycin |
Gibco |
Cat# 15070-063 |
| 1X RBC Lysis Buffer |
Invitrogen |
Cat# 00-4333-57 |
| Rhodamine-conjugated phalloidin |
Molecular Probes |
Cat# R415 |
| RNase-free DNase |
QIAGEN |
Cat# 79254 |
| Tamoxifen |
Toronto Research Chemicals |
Cat# T006000 |
| TRIzol® Reagent |
Ambion |
Cat# 15596018 |
| Critical commercial assays |
| Click-iT™ EdU Alexa Fluor™ 488 Imaging Kit |
Thermo Fisher |
Cat# C10337
|
| Maxima First Strand cDNA Synthesis Kit |
Thermo Scientific |
Cat# K1641 |
| RNeasy Mini Kit |
QIAGEN |
Cat# 74104 |
| RNeasy Plus Mini Kit |
QIAGEN |
Cat# 74134 |
| RNeasy Plus Micro Kit |
QIAGEN |
Cat# 74034 |
| TSA Plus Cyanine 3 (Cy3) Fluorescein detection kit |
Perkin-Elmer |
Cat# NEL753001KT |
| Two-well culture insert |
Ibidi |
Cat# 80209 |
| Deposited data |
| Bulk RNA-Seq data for peripheral lungs of PdgfraH2BGFP mice at P3 |
This paper |
GSE189466 |
| scRNA-Seq data for peripheral lungs of control mice at P3 |
This paper |
GSE189466 |
| Experimental models: Cell lines |
| Primary fibroblasts derived from peripheral lungs of control mice at P3 |
This paper |
N/A |
| Primary neurons derived from the superior cervical ganglion (SCG) of Ptox; Sox10Cre mice at P3 |
This paper |
N/A |
| Primary neurons derived from the superior cervical ganglion (SCG) of Sox10Cre mice at P3 |
This paper |
N/A |
| Primary neurons derived from the superior cervical ganglion (SCG) of Vangl2f/f; Sox10Cre mice at P3 |
This paper |
N/A |
| Rat: PC12 cells |
ATCC |
CRL-1721 |
| Experimental models: Organisms/strains |
| Mouse: Adrb1− [FVB.129-Adrb1tm1Bkk/J] |
The Jackson Laboratory |
Stock# 031490 |
| Mouse: Adrb2− [FVB.129-Adrb2tm1Bkk/J] |
The Jackson Laboratory |
Stock# 031496 |
| Mouse: Adrb3− [FVB/N-Adrb3tm1Lowl/J] |
The Jackson Laboratory |
Stock# 006402 |
| Mouse: BdnfCre [B6.FVB-Bdnfem1(cre)Zak/J] |
The Jackson Laboratory |
Stock# 030189 |
| Mouse: Bdnff [STOCK Bdnftm3Jae/J] |
The Jackson Laboratory |
Stock# 004339 |
| Mouse: ChatCre [B6;129S6-Chattm2(cre)Lowl/J] |
The Jackson Laboratory |
Stock# 006410 |
| Mouse: GCaMP6f [B6;129S-Gt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze/J] |
The Jackson Laboratory |
Stock# 024105 |
| Mouse: Ngff
|
Dr. Liliana Minichiello |
N/A |
| Mouse: NgfLacZ [Ngftm1Ddg] |
Dr. David Ginty |
N/A |
| Mouse: PDGFRaCre [C57BL/6-Tg(Pdgfra-cre)1Clc/J] |
The Jackson Laboratory |
Stock# 013148 |
| Mouse: PDGFRaCreER [B6N.Cg-Tg(Pdgfra-cre/ERT)467Dbe/J] |
The Jackson Laboratory |
Stock# 018280 |
| Mouse: PDGFRaH2BGFP [B6.129S4-Pdgfratm11(EGFP)Sor/J] |
The Jackson Laboratory |
Stock# 007669 |
| Mouse: RC::PFtox [B6;129S6-Gt(ROSA)26Sortm8(CAG-mCherry,-EGFP)Dym/J] |
The Jackson Laboratory |
Stock# 029486 |
| Mouse: Ror2f [B6;129S4-Ror2tm1.1Meg/J] |
The Jackson Laboratory |
Stock# 018354 |
| Mouse: ROSA26mTmG [B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J] |
The Jackson Laboratory |
Stock# 007676 |
| Mouse: R26R [B6.129S4-Gt(ROSA)26Sortm1Sor/J] |
The Jackson Laboratory |
Stock# 003474 |
| Mouse: ROSA26tdTomato [B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J] |
The Jackson Laboratory |
Stock# 007914 |
| Mouse: Sox10Cre [B6;CBA-Tg(Sox10-cre)1Wdr/J] |
The Jackson Laboratory |
Stock# 025807 |
| Mouse: Sox10iCreER [CBA;B6-Tg(Sox10-icre/ERT2)388Wdr/J] |
The Jackson Laboratory |
Stock# 027651 |
| Mouse: ThCre [B6;129X1-Thtm1(cre)Te/Kieg] |
Dr. Ted Ebendal |
N/A |
| Mouse: TrkAf [B6.129P2(SJL)-Ntrk1tm1Ddg/J] |
The Jackson Laboratory |
Stock# 022362 |
| Mouse: TrkBf [Ntrk2tm1.1Lfr] |
Dr. Baoji Xu |
N/A |
| Mouse: Vangl1gt [Vangl1GT(XL802)Byg] |
Dr. Philippe Gros |
N/A |
| Mouse: Vangl2f [Vangl2tm1.1Yy] |
Dr. Yingzi Yang |
N/A |
| Mouse: Wnt1Cre [H2az2Tg(Wnt1-Cre)11Rth] |
The Jackson Laboratory |
Stock# 003829 |
| Mouse: Wnt1Cre2 [129S4.Cg-E2f1Tg(Wnt1-cre)2Sor/J] |
The Jackson Laboratory |
Stock# 022137 |
| Oligonucleotides |
| qPCR primer: Mus musculus Chat (fwd): 5’-GCCATTGTGAAGCGGTTTG-3’ |
This paper |
N/A |
| qPCR primer: Mus musculus Chat (rev): 5’-TCATTCAGCCAGTATTCAGAGAC-3’ |
This paper |
N/A |
| qPCR primer: Mus musculus Gapdh (fwd): 5’-AGGTTGTCTCCTGCGACTTCA-3’ |
This paper |
N/A |
| qPCR primer: Mus musculus Gapdh (rev): 5’-CCAGGAAATGAGCTTGACAAAGTT-3’ |
This paper |
N/A |
| qPCR primer: Mus musculus Th (fwd): 5’-AAGATCAAACCTACCAGCCG-3’ |
This paper |
N/A |
| qPCR primer: Mus musculus Th (rev): 5’-TACGGGTCAAACTTCACAGAG-3’ |
This paper |
N/A |
| qPCR primer: Mus musculus VAChT (fwd): 5’-CGTATCAGTCTATGGCAGTGTC-3’ |
This paper |
N/A |
| qPCR primer: Mus musculus VAChT (rev): 5’-TGAGTGAACGATATGGCCTG-3’ |
This paper |
N/A |
| qPCR primer: Homo sapiens CHAT (fwd): 5’-GCCCTGCCGTGATCTTTG-3’ |
This paper |
N/A |
| qPCR primer: Homo sapiens CHAT (rev): 5’-GCCTTGTAGCTGAGTACACC-3’ |
This paper |
N/A |
| qPCR primer: Homo sapiens DBH (fwd): 5’-ACGTACTGGTGCTACATTAAGG-3’ |
This paper |
N/A |
| qPCR primer: Homo sapiens DBH (rev): 5’-TTGCCCTTGGTGACGATG-3’ |
This paper |
N/A |
| qPCR primer: Homo sapiens GAPDH (fwd): 5’-CTGACTTCAACAGCGACACC-3’ |
This paper |
N/A |
| qPCR primer: Homo sapiens GAPDH (rev): 5’-TAGCCAAATTCGTTGTCATACC-3’ |
This paper |
N/A |
| qPCR primer: Homo sapiens NPY (fwd): 5’-AAAGCACAGAAAATGTTCCCAG-3’ |
This paper |
N/A |
| qPCR primer: Homo sapiens NPY (rev): 5’-GCTGAAAATAGGAAAAGGCCAG-3’ |
This paper |
N/A |
| qPCR primer: Homo sapiens TH (fwd): 5’-CCGTGCTAAACCTGCTCTTC-3’ |
This paper |
N/A |
| qPCR primer: Homo sapiens TH (rev): 5’-GGTGGATTTTGGCTTCAAACG-3’ |
This paper |
N/A |
| qPCR primer: Homo sapiens VIP (fwd): 5’-TTCTCACAGACTTCGGCATG-3’ |
This paper |
N/A |
| qPCR primer: Homo sapiens VIP (rev): 5’-TCAGGTTCATTTGCTCCCTC-3’ |
This paper |
N/A |
| Recombinant DNA |
|
|
|
| Software and algorithms |
| ImageJ |
N/A |
https://imagej.nih.gov/ij/
|
| Prism 5.0 |
GraphPad |
https://www.graphpad.com/
|
| RStudio |
RStudio® |
https://www.rstudio.com/
|
| Other |
|
|
|
