Table 6.
PCR amplification reaction procedure.
| Temperature (°C) | Lasting time | Cycle (n) |
|---|---|---|
| 95 | 3 min | 1 |
| 95 | 30 s | 27 |
| 55 | 30 s | 27 |
| 72 | 45 s | 27 |
| 72 | 10 min | 1 |
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