Table 2.
The gene primer sequences used for RT-qPCR.
| Gene name | Forward primer (5′->3′) | Reverse primer (5′->3′) |
|---|---|---|
| ADH1 | TGGGAAAAGTATCCGTACCATT | AGCTGTTGCTCCAGATCATGT |
| ADH2 | GGGCAGAGAAGACAGAAACGA | TTCCTCTCATCAAACTTCTGTCA |
| ADH3 | CCCAAACTTGTGGCTGACTTT | ACTCTTTCCAGAGCGAAGCA |
| CYP2E1 | GAAGCCTCTCGTTGACCCAA | TGGTGGGATACAGCCAAACC |
| ALDH2 | CATGGACGCATCACACAGGG | CTTGCCATTGTCCAGGGTCT |
| COXIV | GGCGGCAGGTGTACATTTTTA | AGTCTTCGCTCTTCACAACA |
| VDAC | CCCAAACTTGTGGCTGACTTT | ACTCTTTCCAGAGCGAAGCA |
| PINK1 | AACCGCTTCGACTTTCTGCT | CACTTGATGAACCAGCCCCA |
| Mfn1 | TGGCCACATGTAGTTTATGTTTC | TTGCACCTGCTGTAAAAAGGC |
| Mfn2 | GGAAGGTGAAGCGCAATGTC | TGCATTCACCTCAGCCATGT |
| Opa1 | CTGTGGCCTGGATAGCAGAA | AGACTGGCAGACCTCACTCT |
| Drp1 | CCGGAGACCTCTCATTCTGC | TCTGCTTCCACCCCATTTTCT |
| Fis1 | TCAGCCCCATCATGTGCTTT | CAGGAGAGGACCAGGAGTGA |
| Mff | CTCTCAGCCAACCACCTCTG | GTGCTGGATTGAGAGCCACT |
| TWNK | CTGGTTGGGGGATGCCTTC | ATTGAAGCCTCCGTTCAGGG |
| MFND | CAACTACGCAAAGGCCCCA | TGATGGTAGATGTGGCGGGT |
| MTCO1 | CTATCCGGAATGCCCCGA | GGCATCCATATAGTCACTCCAG |
| IL-18 | TGCAGTCTACACAGCTTCGG | GCAGCCATCTTTATTCCTGCG |
| IL-1β | TGATGGCTTATTACAGTGGCA | CGGAGATTCGTAGCTGGATG |
| Caspase-1 | CCTGCCGTGGTGATAATGTT | TCCACATCACAGGAACAGGC |
| GSDMD | CAGAAGGGACGTGGTGTTCC | AGTTTACGGAAGTCGGCGAG |
| GAPDH | ACTAGGCGCTCACTGTTCT | CCAATACGACCAAATCCGTTG |