Fig. 2.
I/R-induced BUMPT cell apoptosis was ameliorated by lncRNA ENSMUST_147219 siRNA. BUMPT cells were transfected with 100 nM lncRNA ENSMUST_147219 siRNA or scramble and then treated with or without I(2 h)/R(2 h). A RT-qPCR analysis of the expression levels of ENSMUST_147219. B and C The FCM analysis of BUMPT cells apoptosis. D Immunoblot blot analysis of cleaved caspase-3 and caspase-3. E Densitometric measurement of immunoblot blot bands. Data are expressed as mean ± SD (n = 6). #P < 0.05, versus scramble with saline group; *P < 0.05, versus scramble with I/R group