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. 2022 May 26;27(7-8):531–544. doi: 10.1007/s10495-022-01730-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — I/R-induced BUMPT cell apoptosis was reinforced by overexpression of lncRNA ENSMUST_147219. A BUMPT cells were transfected with lncRNA ENSMUST_147219 plasmid or control and then treated with or without I(2 h)/R(2 h). RT-qPCR analysis of the expression levels of ENSMUST_147219. B and C The FCM analysis of BUMPT cells apoptosis. D Immunoblot blot analysis of cleaved caspase-3 and caspase-3. E Densitometric measurement of immunoblot blot bands. Data are expressed as mean ± SD (n = 6). ^#P < 0.05, versus scramble with saline group; *P < 0.05, versus scramble with I/R group