Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 26;27(7-8):531–544. doi: 10.1007/s10495-022-01730-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — Knockdown of lncRNA ENSMUST_147219 reduced I/R-induced BUMPT cell apoptosis, which was reversed by the miR-221-5p inhibitor. BUMPT cells were co-transfected with ENSMUST_147219 siRNA (100 nM) and anti-miR-221-5p or scramble and then subjected to I(2 h)/R(2 h). A RT-qPCR analysis of the expression levels of ENSMUST_147219. B RT-qPCR analysis of the expression levels of miR-221-5p. C and D The FCM analysis of BUMPT cells apoptosis. E Immunoblot analysis of cleaved caspase-3 and caspase-3. F Densitometric analysis of immunoblot bands. Data are expressed as mean ± SD (n = 6). ^#P < 0.05, scramble or lncRNA ENSMUST_147219 siRNA with I/R2-2 group versus scramble group; *P < 0.05, lncRNA ENSMUST_147219 siRNA plus anti-miR-221-5p with I/R2-2 group versus lncRNA ENSMUST_147219 siRNA with I/R2-2 group