Fig. 8.

LncRNA ENSMUST_147219 siRNA attenuated I/R-induced AKI in male C57BL/6 mice by targeting the miR-221-5p/IRF6 axis. C57BL/6J mice were preinjected with lncRNA ENSMUST_147219 siRNA or control siRNA via the tail vein for 12 h before ischemic injury, and the blood samples and kidney samples were collected at 48 h after reperfusion. Blood samples were collected for the measurement of serum creatinine (A) and serum nitrogen (BUN) (B) concentration. The sections of kidney were stained with hematoxylin and eosin (H&E) (C) and TUNEL (D). E Tubular damage scores of the kidney. F Counting of TUNEL-positive cells. G and H RT-qPCR analysis of the expression levels of ENSMUST_147219 and miR-221-5p. I Immunoblot analysis of cleaved caspase-3, caspase-3, IRF6, and β-tubulin. J Densitometric analysis of immunoblot bands. Data are expressed as mean ± SD (n = 6). ^#P < 0.05, scramble with IR48h group or lncRNA ENSMUST_147219 siRNA group versus saline group; *P < 0.05, lncRNA ENSMUST_147219 siRNA with IR48h group versus control with IR48h group. Original magnification, × 200