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Ouchterlony agar-gel immunodiffusion. Carbohydrate antigen extracts of Dan 1 and Dan 4 early isolates (wells 1 and 2, respectively), and KFP 173 and KFP 186 recent isolates (wells 3 and 4, respectively), and the fish pathogen Lactococcus garvieae ATCC 43921T (well 5, negative control) were reacted with anti-Dan 1 serum (central well).
