Table 3.
Phenotypic characteristics which distinguish isolate MGRD01-02T from its closest phylogenomic neighbours
| Characteristics | Strains | |||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | |
| API-ZYM tests | ||||||
| Acid and alkaline phosphatases, α-glucosidase | + | + | + | + | + | − |
| α-Chymotrypsin, trypsin | − | − | + | + | + | − |
| Cystine arylamidase | − | − | + | + | − | − |
| Esterase (C4), esterase lipase (C8) β-glucosidase | + | + | + | + | − | − |
| α- and β-Galactosidase, leucine and valine arylamidases | − | + | + | + | + | − |
| β-Glucuronidase | − | + | + | + | − | − |
| Lipase (C14) | − | + | + | − | − | − |
| α-Mannosidase | − | + | − | + | − | − |
| Biochemical tests | ||||||
| Nitrate reduction | + | + | + | − | + | − |
| Oxidase | + | + | + | + | + | − |
| Degradation tests (% w/v) | ||||||
| Hypoxanthine (0.4) | + | − | − | − | − | − |
| Starch (1) | − | + | − | − | + | + |
| Tween 20 (1) | − | − | + | − | − | + |
| Tweens 40 and 60 (1) | + | + | + | + | − | − |
| Xanthine, xylan (0.4) | − | − | − | − | + | + |
| Nutritional tests | ||||||
| Sole carbon sources (1%, w/v) | ||||||
| Inulin | + | − | − | − | − | − |
| d-Mannitol | − | − | − | − | + | − |
| d-Raffinose | + | + | − | + | + | + |
| d-Sucrose | − | − | − | − | + | − |
| d-Trehalose | + | + | + | − | + | + |
| Sole nitrogen sources (1%, w/v) | ||||||
| Acetamide, l-alanine | − | + | − | − | + | + |
| l-Isoleucine, l-valine | − | + | + | − | − | − |
| l-Phenylalanine | − | − | + | − | − | + |
| Tolerance tests | ||||||
| Growth in presence of NaCl (%, w/v) | 0–1 | 0–1 | 0–1 | 0 | 0 | 1–3 |
| pH range | 4.5–6.5 | 4.5–6.0 | 4.0–6.0 | 4.8–6.0 | 3.5–6.5 |
6.0– 10.0 |
|
Optimal pH Temperature range (°C) Optimal temperature (°C) |
5.5 20–45 37 |
5.0 20–37 28 |
5.5 10–45 28 |
5.5 10–37 22–28 |
5.5 10–45 25 |
5.0 10–37 22–28 |
Strains: 1. Isolate MGRD01-02T, 2. Actinospica acidiphila NRRL B-24431T, 3. Actinospica durhamensis CSCA 57T, 4. Actinospica robiniae DSM 44926T, 5. Actinocrinis puniceicyclus DSM 45168T, 6. Catenulispora acidiphila DSM 44928T. +, positive, −, negative. The strains were positive for naphthol-AS-BI-phosphohydrolase (API-ZYM test), produced was H2S and used d-glucose as a sole carbon source, but did not form α-fucosidase (API-ZYM test), reduce nitrite, use acetate, benzoate, fumarate, pyruvate or succinate (sodium salts) as sole carbon sources or degrade adenine (0.5w/v), casein (1), chitin (0.4), elastin (0.3), gelatin (1), guanine (0.3), uric acid (0.4), Tween 80 (1) or l-tyrosine (0.4). The optimal pH and temperatures of the strains ranged from pH 5.0–5.5 and from 25 to 28 °C