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. Author manuscript; available in PMC: 2023 Jul 8.
Published in final edited form as: Circ Res. 2022 May 25;131(2):e34–e50. doi: 10.1161/CIRCRESAHA.121.319976

Figure 2.

Figure 2.

Preventing Ser205 phosphorylation rescues APN cytoprotective effect. 24 hours after being transfected with 3xFlag-hAdipoR1WT or hAdipoR1S205A, NMVMs isolated from adipoR1 knockout mice were infected with either Ad-empty or Ad-GRK2 vector. 48 hours later, cells were treated with APN (2 μg/mL) for 24h followed by H2O2 for 2h. A/B: NMVMs viability was evaluated by MTT assay (n=8 for A and n=6 for B). C/D: NMVMs injuries were determined by LDH assay (n=7 for C and n=6 for D). E-H: Western blot was performed to analyze protein expression of cleaved caspase 3 and caspase 3 in NMVMs (n=6). Statistical significance was evaluated by a one-way ANOVA. All pairwise comparisons were made. Tukey tests were used to correct for multiple comparisons. Ns indicates not significant.