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. Author manuscript; available in PMC: 2023 Jul 8.
Published in final edited form as: Circ Res. 2022 May 25;131(2):e34–e50. doi: 10.1161/CIRCRESAHA.121.319976

Figure 5.

Figure 5.

GRK2 promoted AdipoR1 degradation via lysosomal degradation in a time-dependent manner. A: Western blot was performed to analyze AdipoR1 protein expressions in AdipoR1-KO/hAdipoR1WT NMVMs infected with Ad-GRK2 for a different time (n=5). B: qPCR was used to determine AdipoR1 mRNA levels in AdipoR1-KO/hAdipoR1WT NMVMs infected with Ad-GRK2 for a different time (n=5). For A and B, statistical significance was evaluated by a Kruskal-Wallis test. Post hoc pairwise tests for indicated group pairs were performed after Dunn correction. Ns indicates not significant. C: The expressions of AdipoR1 by Western blot in AdipoR1-KO/hAdipoR1WT cardiomyocytes infected with Ad-GRK2 for different time and treated with vehicle, MG-132 (5 μg/ml) or chloroquine (200 μM). D: Quantification of Western blot (n=5 for Vehicle, n=4 for MG-132, n=5 for Chloroquine group). Statistical significance was evaluated by a Kruskal-Wallis test within each time point, and all pairwise comparisons were made. Dunn tests were used to correct for multiple comparisons. *p<0.05 Chloroquine vs. Vehicle; #p<0.05 Chloroquine vs. MG-132.