Major Resources Table.
In order to allow validation and replication of experiments, all essential research materials listed in the Methods should be included in the Major Resources Table below. Authors are encouraged to use public repositories for protocols, data, code, and other materials and provide persistent identifiers and/or links to repositories when available. Authors may add or delete rows as needed.
| Animals (in vivo studies) | ||||
|---|---|---|---|---|
| Species | Vendor or Source |
Background Strain | Sex | Persistent ID / URL |
| Mus musculus | MMRRC | B6.129P2-Adipor1tm1Dgen, C57BL/6J | F and M | MMRRC Strain #11599, https://www.mmrrc.org/catalog/sds.php?mmrrc_id=11599 |
| Genetically Modified Animals | |||||
|---|---|---|---|---|---|
| Species | Vendor or Source |
Background Strain |
Other Information | Persistent ID / URL | |
| Parent - Male | |||||
| Parent - Female | |||||
| Antibodies | |||||
|---|---|---|---|---|---|
| Target antigen | Vendor or Source |
Catalog # | Working concentration |
Lot # (preferred but not required) |
Persistent ID / URL |
| Anti-phospho-ERK1/2 rabbit monoclonal antibody | Cell Signaling Technology | 4370 | 1:2000 | https://www.cellsignal.com/product/productDetail.jsp?productId=4370 | |
| Anti-ERK1/2 rabbit monoclonal antibody | Cell Signaling Technology | 4695 | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=4695 | |
| Anti-phospho-AMPKα rabbit polyclonal antibody | Cell Signaling Technology | 2531 | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=2531 | |
| Anti-AMPKα rabbit monoclonal antibody | Cell Signaling Technology | 2603 | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=2603 | |
| Anti-phospho-ACC rabbit monoclonal antibody | Cell Signaling Technology | 11818 | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=11818 | |
| Anti-ACC rabbit polyclonal antibody | Cell Signaling Technology | 3662 | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=3662 | |
| Anti-cleaved caspase-3 rabbit polyclonal antibody | Cell Signaling Technology | 9661 | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=9661 | |
| Anti-caspase-3 rabbit polyclonal antibody | Cell Signaling Technology | 9662 | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=9662 | |
| Anti-phospho-AP2M1 rabbit monoclonal antibody | Cell Signaling Technology | 7399 | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=7399 | |
| Anti-AP2M1 rabbit monoclonal antibody | Abcam | ab75995 | 1:2000 | https://www.abcam.com/ap2m1-antibody-ep2695y-ab75995.html | |
| Anti-GRK2 rabbit polyclonal antibody | Cell Signaling Technology | 3982s | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=3982s | |
| Anti-adipoR1 mouse monoclonal antibody | Santa Cruz Biotechnolog y | sc-518030 | 1:500 | https://www.scbt.com/p/adipor1-antibody-d-9?productCanUrl=adipor1-antibody-d-9&_requestid=5867389 | |
| Anti-β-arrestin1/2 rabbit monoclonal antibody | Cell Signaling Technology | 4674 | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=4674 | |
| Anti-GFP rabbit polyclonal antibody | Genscript | A01704 | 1:500 | https://www.genscript.com/antibody/A01704-THE_GFP_Antibody_pAb_Rabbit.html | |
| Anti-β-actin mouse monoclonal antibody | Santa Cruz Biotechnolog y | sc-47778 | 1:200 | https://www.scbt.com/p/beta-actin-antibody-c4?requestFrom=search | |
| Anti-GAPDH rabbit monoclonal antibody | Cell Signaling Technology | 2118 | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=2118 | |
| Anti-flag M2 rabbit monoclonal antibody | Sigma-Aldrich | F1804 | 1:2000 | https://www.sigmaaldrich.com/US/en/product/sigma/f1804 | |
| Anti-Rab5 rabbit monoclonal antibody | Cell Signaling Technology | 3547 | 1:200 | https://www.cellsignal.com/product/productDetail.jsp?productId=3547 | |
| Anti-LAMP2 rabbit polyclonal Antibody | Bioss | BS-2379R | 1:100 | https://www.biossusa.com/products/bs-2379r | |
| Anti-p-Ser/Phosphoserine mouse monoclonal antibody | Santa Cruz Biotechnology | sc-81514 | 1:1000 | https://www.scbt.com/p/p-ser-antibody-16b4 | |
| Secondary HRP-conjugated anti-mouse antibody | Cell Signaling Technology | 7076 | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=7076 | |
| Secondary HRP-conjugated anti-rabbit antibody | Cell Signaling Technology | 7074 | 1:1000 | https://www.cellsignal.com/product/productDetail.jsp?productId=7074 | |
| Goat anti-Mouse IgG (H+L), Alexa Fluor 546 | ThermoFisher Scientific | A-11003 | 1:500 | https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgG-HL-Cross-Adsorbed-Secondary-AntibodyPolyclonal/A-11003 | |
| Goat Anti-Rabbit IgG H&L (Cy5) | Abcam | Ab6564 | 1:2000 | https://www.abcam.com/goat-rabbit-igg-hl-cy5--preadsorbed-ab6564.html#minReactivity | |
| DNA/cDNA Clones | |||
|---|---|---|---|
| Clone Name | Sequence | Source / Repository | Persistent ID / URL |
| 3xFlag-hAdipoR1 | Human AdipoR1 cDNA | This paper/In house | |
| AAV9-cTNT-eGFP plasmid | Available at URL | A gift from Dazhi Wang | https://www.addgene.org/105543/ |
| AAV helper plasmid pAdDeltaF6 | Available at URL | A gift from Dazhi Wang | https://www.addgene.org/112867/ |
| AAV packaging plasmid pAAV2/9n | Available at URL | A gift from Dazhi Wang | https://www.addgene.org/112865/ |
| pSNAPf vector | Available at URL | New England Biolabs | https://www.neb.com/products/n9183-psnapf-vector#Product%20Information |
| Cultured Cells | |||
|---|---|---|---|
| Name | Vendor or Source | Sex (F, M, or unknown) | Persistent ID / URL |
| Primary adult cardiomyocyte | Primary cells | F and M | |
| Primary neonatal mouse ventricular myocyte | Primary cells | F and M | |
| Data & Code Availability | ||
|---|---|---|
| Description | Source / Repository | Persistent ID / URL |