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. Author manuscript; available in PMC: 2023 Jan 13.
Published in final edited form as: Nature. 2022 Jul 13;607(7919):563–570. doi: 10.1038/s41586-022-04949-x

Fig 2 |. E. gallinarum liver isolates are primarily derived from lineages associated with the intestinal mucosa.

Fig 2 |

a, Reconstruction of the phylogenetic history of E. gallinarum isolates sampled from mouse 7. Circles represent sequenced isolates and squares represent hypothetical intermediate genotypes. Arrows connect related genotypes and dashed lines connect genetically identical isolates. Liver and faecal populations are separated by a blue dashed line. b, c, Liver clearance of intravenously injected E. gallinarum liver isolates EGmono6-LV1 (manY/lacE/ypdA mutant) and EGmono6-LV10 (immR mutant). See Supplementary Table 7 for a summary of all mutants. Bacterial load of EGmono6-LV1 (b) and EGmono6-LV10 (c) recovered from livers 3 days or 2 weeks post-injection. n = 5 mice. Representative of three independent experiments. Data represent mean ± SEM. d, Schematic of metagenomic sequencing of E. gallinarum populations across tissue sites in monocolonised mice. e, Allele frequency for select mutations across tissue sites. Rows represent mice and columns represent tissue sites. Crosses represent missing values.