Figure 1. Identification of Acj6-regulated mRNAs and cell-surface proteins.

(A) Central question: how does a transcription factor (TF) control targeting specificity of different neuron types by regulating the expression of cell-surface proteins (CSPs)?

(B) Diagram of cell body locations and glomerular innervation patterns of Drosophila olfactory projection neurons (PNs) derived from either the anterodorsal (adPN, colored in blue) or the lateral (lPN) neuroblast lineage. Acj6 is specifically expressed in adPNs, which target dendrites to a stereotyped subset of glomeruli (colored in blue).

(C and D) Compared to those of wild-type (C), dendrites of acj6^−/− adPN neuroblast clones (D) exhibit both loss of innervation (circled in red) and ectopic targeting (circled in white) at a few specific glomeruli. Blue, anti-NCad antibody staining revealing glomerular organization of the antennal lobe. Green, anti-GFP staining of membrane-targeted GFP expressed by PNs.

(E) Schematic of RNA sequencing in developing wild-type (WT) and acj6 mutant adPNs. APF, after puparium formation.

(F) Top five KEGG pathways enriched among genes that are differentially expressed (DE; adjusted p < 0.05) between wild-type and acj6 mutant adPNs.

(G) Schematic of PN surface proteomic profiling in developing wild-type and acj6 mutant brains.

(H) Neutravidin staining of developing PNs expressing HRP after the cell surface biotinylation reaction. Signals are absent intracellularly in PN somata.

Scale bars, 20 μm. D, dorsal; L, lateral.

See also Figure S1.