KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat anti-NCad | Developmental Studies Hybridoma Bank | RRID: AB 528121 |
| Chicken anti-GFP | Aves Labs | RRID: AB 10000240 |
| Mouse anti-FLAG | Sigma-Aldrich | RRID: AB 259529 |
| Mouse anti-V5 | Sigma-Aldrich | RRID: AB 2556564 |
| Deposited data | ||
| RNA sequencing data | This paper | GEO: GSE200841 |
| Mass spectrometry proteomics data | This paper | MassIVE: MSV000089187 |
| Experimental models: Organisms/strains | ||
| D. melanogaster: tubP-GAL80 | (Lee and Luo, 1999) | RRID: BDSC_9917 |
| D. melanogaster: UAS-mCD8-GFP | (Lee and Luo, 1999) | RRID: DGRC_108068 |
| D. melanogaster: hsFlp | (Golic and Lindquist, 1989) | N/A |
| D. melanogaster: FRT19A | (Xu and Rubin, 1993) | RRID: BDSC_1744 |
| D. melanogaster: FRT40A | (Xu and Rubin, 1993) | N/A |
| D. melanogaster: FRT42D | (Xu and Rubin, 1993) | RRID: BDSC_1802 |
| D. melanogaster: GH146-Flp | (Hong et al., 2009) | N/A |
| D. melanogaster: acj66 | (Clyne et al., 1999) | RRID: BDSC_60686 |
| D. melanogaster: otk3 | (Winberg et al., 2001) | N/A |
| D. melanogaster: piezoKO | (Kim et al., 2012) | RRID: BDSC_58770 |
| D. melanogaster: VT033006-GAL4 | (Tirian and Dickson, 2017) | RRID: VDRC_202281 |
| D. melanogaster: GH146-GAL4 | (Stocker et al., 1997) | RRID: BDSC_30026 |
| D. melanogaster: VT033006-GAL4DBD | Yoshi Aso (unpublished) | N/A |
| D. melanogaster: C15-p65AD | (Xie et al., 2019) | N/A |
| D. melanogaster: UAS-HRP-CD2 | (Larsen et al., 2003) | RRID: BDSC_9906 |
| D. melanogaster: UAS-RNAi lines | (Dietzl et al., 2007; Ni et al., 2011; Perkins et al., 2015) | Stock numbers listed in Figure S3 |
| D. melanogaster: UAS-dcr2 | (Dietzl et al., 2007) | N/A |
| D. melanogaster: UAS-otk | (Winberg et al., 2001) | N/A |
| D. melanogaster: UAS-piezo | This study | N/A |
| D. melanogaster: UAS-piezo-2306Myc | This study | N/A |
| D. melanogaster: UAS-acj6-J | (Bai and Carlson, 2010) | N/A |
| D. melanogaster: UAS-Dg | Robert Ray, Francis Crick Institute | RRID: BDSC_63052 |
| D. melanogaster: UAS-Dop1R1 | This study | N/A |
| D. melanogaster: UAS-Dop2R | (Deng et al., 2019) | RRID: BDSC_86134 |
| D. melanogaster: UAS-Nep3 | This study | N/A |
| D. melanogaster: UAS-CG5027 | This study | N/A |
| D. melanogaster: UAS-Ostγ | This study | N/A |
| D. melanogaster: otk-FRT-V5-STOP-FRT-FLAG-STOP | This study | N/A |
| D. melanogaster: piezo-FRT-V5-STOP-FRT-FLAG-STOP | This study | N/A |
| D. melanogaster: Dg-FRT-V5-STOP-FRT-FLAG-STOP | This study | N/A |
| HEK-PI KO cells | (Lukacs et al., 2015) | N/A |
| Recombinant DNA | ||
| Conditional tag cassette FRT-1xV5-6xStop-loxP-mini-White-loxP-FRT-3xFLAG-6xStop | (Li et al., 2020) | N/A |
| pCR-Blunt-TOPO | Thermo Fisher Scientific | Catalog #: K280020 |
| pU6-BbsI-chiRNA | (Gratz et al., 2014; Gratz et al., 2013) | RRID: Addgene_45946 |
| pUASTattB | (Bischof et al., 2007) | RRID: DGRC_1419 |
| pUAST-piezo-901Myc-FLAG | This study | N/A |
| pUAST-piezo-2289Myc-FLAG | This study | N/A |
| pUAST-piezo-2291Myc-FLAG | This study | N/A |
| pUAST-piezo-2306Myc-FLAG | This study | N/A |
| pcDNA3.2-piezo-IRES2-mNeonGreen | Ardem Patapoutian, Scripps Research Institute | N/A |
| pcDNA3.2-piezo-WT-IRES2-mNeonGreen | This study | N/A |
| pcDNA3.2-piezo-2289Myc-mNeonGreen | This study | N/A |
| pcDNA3.2-piezo-2291Myc-mNeonGreen | This study | N/A |
| pcDNA3.2-piezo-2306Myc-mNeonGreen | This study | N/A |
| Software and algorithms | ||
| Zen | Carl Zeiss | RRID: SCR_013672 |
| ImageJ | National Institutes of Health | RRID: SCR_003070 |
| Illustrator | Adobe | RRID: SCR_010279 |
| Spectrum Mill | Agilent | https://www.agilent.com/Agilent404?s=https://www.agilent.com/en/products/software-informatics/masshunter-suite/masshunter-for-life-science-research/spectrum-mill |
| pClamp | Molecular Devices | RRID: SCR_011323 |