(A) Circulating 12,13-diHOME levels in plasma of vehicle-treated or AngII-treated mice after 7 days of exposure at 23°C or 5°C for 7 days in the absence or the presence of hydralazine. (B) Plasma 12,13-diHOME concentrations in ETBRKO mice (Left) and eNOSKO mice (Right) at 23°C or 5°C for 7 days. (C) Plasma 12,13-diHOME levels in L-NAME treated mice or AngII treated mice after cold exposure 7days. (D) Plasma Nitrite/Nitrate levels in vehicle-treated or AngII-treated mice after cold exposure. (E) Plasma Nitrite/Nitrate levels in ETBRKO mice (Left) and eNOSKO mice (Right) at 23°C or 5°C for 7 days. (F) Plasma Nitrite/Nitrate levels in L-NAME treated mice or AngII treated mice after cold exposure. Biological replicates n=3-4 for each group. P values were determined by Kruskal-Wallis with Dunn’s multiple comparison test in A, B, C, D, E and F. Data presented as mean ± SD; n=mice/group as indicated.