Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-NeuN antibody | Abcam | Cat #ab177487; RRID:AB_2532109 |
| Chicken anti-PGP9.5 antibody | Invitrogen | Cat #PA1–10011; RRID:AB_1088162 |
| Goat anti-chicken Alexa647 antibody | Invitrogen | Cat #A32933; RRID: AB_2762845 |
| Donkey anti-rabbit Alexa555 antibody | Invitrogen | Cat #A21432; RRID: AB_2535853 |
| Rabbit anti-CGRP antibody | Millipore | Cat #PC205L; RRID: AB_2068524 |
| Rabbit anti-Neurofilament 200 antibody | Sigma | Cat #N4142; RRID: AB_477272 |
| Mouse anti-Tuj1antibody | Abcam | Cat #ab7751; RRID: AB_306045 |
| Donkey anti-rabbit DyLight488 antibody | Abcam | Cat #ab96919; RRID: AB_10679362 |
| Donkey anti-mouse Alexa647 antibody | Abcam | Cat #ab150107; RRID: AB_2890037 |
| Rabbit anti-dsRed antibody | Takara Bio | Cat #632496; RRID: AB_10013483 |
| Mouse anti-Neurofilament 200 antibody | Sigma-Aldrich | Cat #N0142; RRID: AB_477257 |
| Goat anti-calcitonin gene-related peptide antibody | Bio-Rad | Cat #1720–9007; RRID: AB_2290729 |
| Goat anti-TRPV1 antibody | Neuromics | Cat #GT15129; RRID: AB_1624144 |
| Donkey anti-rabbit AF594 antibody | Jackson Immuno | Cat #711-585-152; RRID: AB_2340621 |
| Donkey anti-mouse AF488 antibody | Jackson Immuno | Cat #715-545-150; RRID: AB_2340846 |
| Donkey anti-goat AF488 antibody | Jackson Immuno | Cat #705-545-147; RRID: AB_2336933 |
| Mouse anti-NeuN antibody | Chemicon | Cat #MAB377; RRID: AB_2298772 |
| Rabbit anti-S100 beta antibody | Abcam | Cat #ab52642; RRID: AB_882426 |
| Rabbit anti-Parvalbumin antibody | Abcam | Cat #ab181086; RRID: N/A |
| Rabbit anti-Olig2 antibody | Abcam | Cat #ab109186; RRID: AB_10861310 |
| Rabbit anti-GLUT1 antibody | Millipore | Cat #07–1401; RRID: AB_11212210 |
| Goat anti-rabbit Alexa647 antibody | Invitrogen | Cat # A21245, RRID: AB_141775 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J | Jackson Laboratory | Cat #000664 |
| Mouse: Tek-Cre | Jackson Laboratory | Cat #008862 |
| Mouse: ChAT-IRES-Cre | Jackson Laboratory | Cat #006410 |
| Mouse: Nestin-Cre | Jackson Laboratory | Cat #003771 |
| Mouse: TRPV1-Cre | Jackson Laboratory | Cat #017769 |
| Mouse: TH-Cre | European Mouse Mutant Archive | Cat #EM::00254 |
| Sprague-Dawley rat | University of Melbourne | N/A |
| Marmoset | University of California San Diego | N/A |
| Rhesus Macaque | University of California Davis | N/A |
| Recombinant DNA | ||
| pUCmini-iCAP-AAV.MaCPNS1 | In house | Deposited at Addgene #185136 |
| pUCmini-iCAP-AAV.MaCPNS2 | In house | Deposited at Addgene #185137 |
| pAAV:hSyn1-tdTomato plasmid | Addgene | #51506 |
| pAAV:CAG-tdTomato plasmid | Addgene | #59462 |
| pAAV:hSyn-DIO-hM3D(Gq)-mCherry plasmid | Addgene | #44361 |
| pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid | Addgene | #162380 |
| Software and algorithms | ||
| Graphpad Prism v9 | Graphpad Prism Inc | https://www.graphpad.com/scientific-software/prism/ |
| Keyence BZ-X Analyzer | Keyence | https://www.keyence.com/ |
| Zen | Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html |
| Imaris | Oxford Instruments | https://imaris.oxinst.com/ |
| Biorender | Biorender | https://biorender.com/ |
| Adobe Illustrator | Adobe | https://www.adobe.com/products/illustrator/ |
| UMAP analysis code | McInnes, Healy, and Melville 2020 | https://github.com/lmcinnes/umap |
| CaImAn analysis code | Giovannucci et al., 2019 | https://github.com/flatironinstitute/CaImAn-MATLAB |
| Next generation sequencing analysis code | Ravindra Kumar et al., 2020 | https://github.com/GradinaruLab/mCREATE |