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. Author manuscript; available in PMC: 2023 Jul 8.
Published in final edited form as: Circ Res. 2022 Jun 20;131(2):151–164. doi: 10.1161/CIRCRESAHA.122.320785

Figure 3. Supplementation of FGF9 or IGF2 rescues CM proliferation defects.

Figure 3.

(A) Secretion of FGF9 and IGF2 from Hdac3 KO and EV MECs. Coomassie brilliant blue staining of total extracted proteins from supernatants served as protein loading controls. FGF9 and IGF2 in the MEC supernatants were detected by western blot. Arrows point to the target bands. Quantifications are shown on the right. n=5 in each group. (B and C) The effects of MEC supernatants and/or recombinant FGF9 or IGF2 on E13.5 Tnnt2nGFP/+ CM proliferation. Representative immunofluorescence micrographs are shown. Scale bars: 275 μm. Percentage of p-H3+ CMs and total number of CMs were quantitated. Independent samples: FGF9, n=6 in each group; IGF2, n=12 in each group. P-values were determined by the Mann-Whitney U test for (A) and One-way ANOVA followed by the Tukey post hoc test for (B) and (C).