Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 23;13:4257. doi: 10.1038/s41467-022-31938-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Scheme depicting GATA6-driven in vitro differentiation of mES cells used to study PrE specification. b PCA of bulk-RNA-seq at differentiation time-points (solid circles) and single-cell RNA-seq profiles of E3.5 and E4.5 blastocysts (open circles). The PrE precursor state was achieved by 16 h of GATA6-driven differentiation and fully specified PrE-cells appeared at 24-48 h. c Heatmap of transcript z-scores of Epi- and PrE-specific genes shows that most blastocyst lineage-specific genes showed similar changes in expression during GATA6-driven differentiation. d Changes in chromatin accessibility measured by ATAC-seq at PrE and Epi CREs were quickly initiated within 2 h of differentiation. Accessible regions at 48 h were compared to 0 h and classified as up, stable or down. e Median ATAC signal plots depict an increase in ATAC signal at 1033 PrE-CREs and decrease at 479 Epi-Cres. f Browser view at the Sox7 locus showing a progressive loss in ATAC signal at a distal CRE and a gain in accessibility at a proximal CRE. This example shows that not all PrE CREs gained accessibility during PrE-specification.