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. 2022 Jul 23;13:4258. doi: 10.1038/s41467-022-31925-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Blots of membranes of Pv8482 WT and O-antigen mutant probed with unactivated or activated BcpT and detected with affinity-purified α-BcpT. b Blot of purified LPS from the indicated strains probed with Alexa 488 labeled activated BcpT. c Overlay assays of O-antigen mutants of PvCL10 and Bv8482 to purified BcpT. d Agar overlay assays showing the sensitivity of various Bacteroidales species and strains to BcpT. The left most spot of each overlay is 1 µg of toxin and each subsequent spot is a two-fold dilution. e Agar spot overlay assays showing the ability of each of three pBCPT genes to protect PvCL10 from BcpT toxicity when expressed in trans. Overlay assays were performed twice and receptor probing blots were performed twice with consistent results. Source data are provided as a Source Data file.