Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 23;13:4258. doi: 10.1038/s41467-022-31925-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Agar spot overlay assays of four transposon (tn) mutants of PvCL10 that were slightly protected from BcpT and substantial protected from BSAP-3. b Orf map of the anti-sigma factor encoding gene (M098_03760) into which all transposons inserted showing the genetic context of the upstream gene encoding the cognate sigma factor (M0N98_03761). Bottom panel shows the regions of the anti-sigma factor including the inner transmembrane region shown in red and the outer membrane β-barrel region and sites of each of the four transposon mutants. c Agar spot overlay assays showing the sensitivity of the mutant strains of PvCL10 (indicated on right) to each of the toxin-producing strains or the toxin deletion mutants. d Western immunoblot showing LPS O-antigen sizes of various PvCL10 WT and mutant strains (psig and pantisig are plasmids containing the respective gene). The blot was probed with antiserum to PvCL10 O-antigen. e Western immunoblot of whole cell lysates of PvCL10 treated with two concentrations of BcpT for 3 h in duplicate or OD₆₀₀ matched untreated controls. Bacterial equivalent to 4 µl of the culture were added to each well. The blot is probed with antiserum to the PvCL10 O-antigen. f List of the 15 genes that are differentially expressed using the strict cut-off criteria listed in the methods section in PvCL10 tn79 compared to WT PvCL10. Green indicates upregulation and red indicates downregulation. FC represents the fold-change and padj represents the p-value adjusted for multiple comparisons by DESeq2. All 15 genes are also differentially expressed when PvCL10 is treated with BcpT. g Agar spot overlay assays showing the susceptibility of various strains to dilutions of BSAP-3 and BcpT. The first spot of the BSAP-3 plates is 1 µg and the first spot of the BcpT plates is 3 µg and each consecutive spot is a 2-fold dilution left to right on the top row and then left to right on the bottom row. Overlay assays and western immunoblots were performed at least twice with consistent results. Source data are provided as a Source Data file.