Figure 2.

Ouabain reduced virus titer. Vero cells were treated with different concentrations of ouabain before (A,C,E) and after (B,D, 1 h of ZIKV infection with a multiplicity of infection (MOI) of 0.1. After 120 h, the cytophatic effect (E, was observed by optical microscopy and the supernatant was titrated using the TCID₅₀ method. As a positive control, the antiviral compound 6MMPr (60.5 µM) was used in the post-treatment assay. Values were presented as mean ± standard deviation of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey post-test. *P < 0.05, **P < 0.01, ***P< 0.001, ****P < 0.0001 significant in relation to the control.